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. Thermodynamic data on the conformational stability of proteins can be obtained using several chemical or physical perturbants, including chaotropic agents (guanidine hydrochloride, urea), hydrostatic pressure, and temperature
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. RESULTS In this work, we focus on two-state proteins because the hypothesis is that only two well-defined macrostates-native and highly denatured states-are accessible to these systems (67-70)
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. Conversely, P(x) is maximal for small x if one assumes a Flory-style random coil model with uncorrelated fluctuations and uniform (mean-field) chain swelling
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. A local length scale spans five- to nine-residue stretches over which sequence-specific spatial correlations decay
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. In EI^sup sc^ at low pH, the fluorescence intensity of ANS was largely enhanced, and the maximum wavelength of the fluorescence spectrum was 480 nm (Fig
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. , when the CaM:SEF2-1mp interaction brakes, one side of the CaM dimer interaction also momentarily brakes
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. We have found that the emission intensity of labeled coumarin-PLCβ and coumarin-Gβγ showed a substantial and reproducible increase upon the addition of its unlabeled partner, which gives apparent K^sub d^ values similar to those obtained using FRET (Guo et al
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. , 2003a) clearly show that variations in the disk diameter from 9 to 18 nm have no significant effect on the disk stability
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. This is why agitation is important during storage
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. Such findings are of paramount importance for any generalized model of amyloid assembly, as they convey information about structural organization on a subfibrillar level
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. Therefore subsequent stages of hierarchical amyloid formation may be expected to enhance burial of hydrophobic residues and to promote energetically favorable hydrogen bonding
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. brasilianus was found by itself only at three stations
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. The intermolecular interaction involves stable hydrophobic contacts with a unique protruding CD4-Phe43 structure surrounded by an intermolecular hydrogen-bond network that covers the hemisphere of the CD4 D1 domain
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. The melt flow rates of the IPP and EVA polymers versus temperature are listed in Table VI
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. Current models of RNA folding propose distinguishable early and late events during RNA structure formation (Sosnick and Pan, 2003)
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. This collective process could explain the fact that essentially all resonances affected by conformational exchange have approximately the same time constant and are clustered in regions important for either CaM:CaM or CaM:SEF2-1mp interactions
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