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. For comparison, the δ values calculated from native structures are also shown as open circles
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. The lowest protein concentration of 7 µg/ml reveals a large red shift with λ^sub max^ of ~345 nm, whereas protein concentrations of 50 µg/ml and 1 mg/ml result in λ^sub max^-values ~340 nm for the unfolded states
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. To determine whether this could be the case for PLCβ^sub 2^, we monitored the change in emission of CPM-PLCβ^sub 2^ under pressure
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