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As a zinc-dependent enzyme, ACYI is quite stable against denaturation induced by heat stress
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CONCLUSIONS We have used an atomistic EV model, developed in previous work, to show that it is computationally tractable to generate accurate conformational ensembles for proteins in the EV limit
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Fluorescence spectroscopy reports the changes in the tertiary structure of a protein (26)
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6 Hz in the ^sup 1^H and ^sup 15^N dimension, respectively
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For the sample with no added NaCl, the divergence occurs at a mass fraction of ~52% w/w, increasing to 56% w/w at 1M NaCl
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Therefore, we characterized the membrane binding behavior of PLCβ and G-protein subunits before protein-protein association studies
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33 K/min is sigmoidal and reaches a plateau at high temperatures, suggesting that the unfolding transition is complete at 99°C, yet the light-scattering curve recorded in the same experiment does not reach a plateau, and the signal continues to increase upon cooling from 99°C (Fig
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, 2003) of the native structure before protein aggregation have begun to emerge
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Studies on the pathogenic amyloidogenic self-assembly of proteins are showing that different transient morphological forms of aggregates have varying neurotoxicity levels (e
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Relaxation functions in folding kineticsWe first take a very simple case: if the nonnative gutter-stabilizing interactions are perfectly balanced with the entropy changes at each stage of the dimension reduction, then the free-energy profile is itself flat, and the d diffusive dimensions form an effective one-dimensional path along which the folding takes place
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, 1998), as well as the four-way helical junction from which it is derived (F
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The expression of specific adhesion molecules or surface markers may be altered by the culture conditions used during monolayer expansion
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