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Native dimeric GR eluted at a volume corresponding to an apparent molecular weight of ~85 kDa in the calibrated column, indicating a fairly compact dimer structure
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No changes in the elution volume of GR were observed when the sample contained 1 M GdnHCl
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INTRODUCTION The bacterial phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS) is a multiprotein complex system that phosphorylates and, concomitantly, transports carbohydrates across the membrane into the cell (1)
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Samples were incubated at 25°C for 10 min
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RELATIONSHIP TO OTHER MODELSWe stress that the search-space picture explored above is not incompatible with the frameworks or results of the other models discussed in the introduction, but rather serves to show how the apparently alternative models are related, and how high-dimensional features are implicit in all of them
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