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Cold denaturation-induced conformational changes in phosphoglycerate kinase from yeast
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2 K; 4 µM, T^sub m^ = 329
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Complete genome sequence of the model actinomycete Slreplomvces coelicolor A3(2)
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Although suitable data are not available to establish if there is a direct correlation between the above ANS binding capacity and the actual instability to freeze-thawing (depending on the protein, irreversible damage has been estimated by diverse criteria such as loss of enzymatic activity, degree of aggregation, and loss of secondary or tertiary structure and, further, the outcome is generally influenced by the rates of freezing and thawing), it is a common observation that multimeric enzymes tend to be less stable to freezing than their monomeric counterpart (17)
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Mutation studies on E12 (a bHLH protein similar to SEF2-1) have shown a gradual decrease in CaM affinity upon each mutation of an interacting residue, and not the on-off behavior usually seen with lock-and-key or induced-fit binding mechanisms (15)
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Notably, this solution is completely transparent compared to when native TTR is incubated under more mild conditions such as pH 4
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Such work is ongoing in our laboratories, and data from misfolded TTR oligomers recorded from site-specific fluorescence labeling of cysteine mutants have provided size distributions with average rotation correlation times of ~ 150 ns (K
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X-ray crystal structure of lipid-free apoA-2 comprises a bundle of nearly parallel helices that opens upon lipid binding (Kumar et al
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Functional and immunological characterization of human-immunodeficiency-virus type-1 envelope glycoproteins containing deletions of the major variable regions
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Nesting ecology of forestdwelling Great Horned Owls, Bubo virginianus, in the eastern deciduous forest biome
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