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Conversely, the contact density maps for the folded versions of different sequences reflect differences in native-state topologies
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Protein concentration was 4 µM, unless otherwise indicated
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A stressed condition of the native structure in ice appears to be common to most proteins even if oligomers seem to be more susceptible than monomers presumably because the globular fold can be further destabilized by subunit dissociation
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On repeating the process we found a consistent decrease in fluorescence intensity at 300 nm accompanied by an emerging red component in the spectrum and enhanced scattering from the solution, the latter response revealing partial unfolding of the macromolecule with exposure of buried W48 to the solvent and concomitant formation of protein aggregates
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We first analyze the latter class and demonstrate that additional binding of ANS in ice is due to the creation of new binding sites rather than to a more complete saturation of preexisting weak sites after the large freeze-induced concentration of the ligand
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A total of 38-39 vertebrae were recorded in 10-12 mm BL postflexion larvae (n=2)
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However, when a polymer-solvent system undergoes LLPS prior to crystallization in a certain range of polymer concentration (see Figure 1), the crystallization curve is modified
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Moreover, the estimated values of (2) and (3), also displayed in Table 1, were modest: the introductions of Type 1 RP and Type 2 RP were associated with reductions of only 1
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