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. the refolding efficiency after unfolding rSBA with GdnCl was much less, as monitored from fluorescence emission scans
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. The imaging parameters were as follows: field of view = 2
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. , it weighs contributions from all atoms), it cannot be ruled out a priori that soman-inhibition decreases fluctuations in some residues (e
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. Charge neutralization of DNA is a driving force of DNA compaction
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. The compaction of DNA in vivo is achieved by histone proteins, which neutralize ~57% of the DNA negative charge (Morgan et al
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. Indeed, compaction potential appears to be inversely related to the binding activity of monocations
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. We find that Gα(GDP) significantly decreased the volume change for the CPM-PLCβ-Gβγ association (Table 1)
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. We have found that the emission intensity of labeled coumarin-PLCβ and coumarin-Gβγ showed a substantial and reproducible increase upon the addition of its unlabeled partner, which gives apparent K^sub d^ values similar to those obtained using FRET (Guo et al
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. Using the hairpin ribozyme as a model system, we specifically address a key question in RNA folding-how do preformed secondary structure elements dictate the course of subsequent tertiary folding events? Currently, little is known about how secondary structural elements affect the ribozyme conformational distributions important in the course of folding
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