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in communications from Bethany College, an M
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MATERIALS AND METHODS Materials Sodium dodecyl sulfate (SDS) was purchased from Sigma (St
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The protein concentration used for the studies is 0
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These features (namely, the absence of tertiary structure, the lack of a sigmoidal behavior in thermal heating, solvent-exposure of hydrophobic residues, and a large amount of secondary structure) are characteristic of the so-called molten-globule species (47,48)
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Most of these difficulties are not present if λ^sub max^-signals are employed to characterize unfolding transitions, although these signals can give a skewed assignment of the population of microstates, because a priori the λ^sub max^-signal is not proportional to the population of the states
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2-cm cell at a protein concentration of 0
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However, the fluorescence emission peak maxima obtained with a heating rate of 1°C/min exhibit a pronounced dependence on the protein concentration
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0Bi solder is less than that of the conventional Sn-Pb solder, and a higher soldering temperature enhances the diffusion process, resulting in a decrease in contact angles and improvement of the wettability
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The receptor in turn catalyzes the exchange of GTP for GDP on the α-subunit of a heterotrimeric G-protein
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Thus, incubation of intact disks at 75°C for 30 min leads to disk fusion into enlarged particles that form stacks similar to disks but have larger diameters (d ≥ 25 nm) and occasionally form U-shaped bilayer structures typical of collapsed protein-containing vesicles (Fig
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Gantz, and Olga GurskyDepartment of Physiology and Biophysics, Boston University School of Medicine, Boston, MassachusettsSubmitted November 9, 2004, and accepted for publication January 21, 2005
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This is particularly relevant to emulsifiers, some of which have a melting point above 150[degrees]F
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CONSEQUENCES OF HIGH-DIMENSIONAL SEARCHESWe have identified two general predictions of this high-dimensional view of folding: 1), the sequential diffusive exploration of low-dimensional subspaces favored by fast folding; and 2), the suggestive stabilization of these subspaces by discriminate but nonnative (or near-native) interactions, without recourse to long-range guiding forces
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Table 2Monthly ranges for age, length, and maturity stage ofblack rockfish collected off Oregon from March 1996through March 1997 for histological analysis
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