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. People, I feared, were only getting half the dose they needed
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. By using a combination of fluorescence and circular dichroism spectroscopies, thiol group titration, and size-exclusion chromatography we have detected the existence of partially folded intermediate states in the unfolding of GR
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. 02 (Perkin-Elmer) with a γ factor of 2
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. gSBA In a typical preparation, 250 g of soybean seeds were homogenized and defatted
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. Protein concentration used for isothermal melts in lhe fluorimeler was 0
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. Cavity statistics, P^sub oa^(r) folded ubiquitin are shown in Fig
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. ANS-binding was used to monitor the extent of exposure of protein hydrophobia regions
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. Address reprint requests to J
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. 3 A compares the effect of 2 µM BSA on the ANS intensity in supercooled liquid and frozen states at -5°C
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. , no significant differences in global molecular dynamics on an Ångström-nanosecond scale between native and soman-inhibited HuBChE below 334 K (61°C), but increased flexibility of the native sample with respect to its inhibited counterpart above that temperature-be reconciled with the cited studies?Results below 61° CIdentical MSDs of two samples over a temperature range as extended as 20-334 K means that the atoms of both samples are placed in common mean free-energy surfaces (47)
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. Nature has thus found a way to specifically recognize a relatively ill-fitting target
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. As shown, regardless of the PEG length, the order of compaction potential for metal chlorides with the exception of LiCl is as follows:NaCl > KCl > RbCl > CsCl
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. This concentration of Gα^sub s^(GDP) was able to eliminate the 2
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. , 2004), and insulin (Bouchard et al
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. The Levinthal paradox: yesterday and today
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. Flavor remains a dynamic element in ice cream and needs to be managed accordingly
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. [FIGURE 1 OMITTED]The oldest eggs of P
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. The simulations incorporate peak broadening due to interconversion of the proposed extended undocked and quasi-docked conformations (Fig
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. Our representatives should be reminded that the FDA's mission is to serve the public's interest, not those of industry
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. The strategy succeeded: Human-colon tumors vanished in mice receiving the combination treatment, but the cancer continued growing in animals getting either treatment alone, the researchers report in the Nov
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. The position of attachment of each of the glycans ht a monomeric subunit is shown in Fig
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. Measuring the confomiational stability of a protein
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. Apoptosis and formation of peroxynitrite in the lungs of patients with obliterative bronchiolitis
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.As described in the Theory section, k^sub ex^ can be derived from the correlation between R^sub ex^ and ΔR^sub ex^ without prior assumption on the timescale of the exchange
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. All the significantly exchanging residues are clustered in well-defined regions, namely on the CaM: SEF2-1 mp interaction interface, in the CaM:CaM dimerization interface or in the flexible hinge region that connects the N- and C-terminal domains within the CaM monomer
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. An alternative explanation is that in this concentration range, the presence of an interfacial layer of protein has a marked influence on the observed rheology
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. Both types of G-protein subunits were destabilized under pressure, suggesting an inefficient packing with the membrane surface
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. For example, curvature in the "Chevron plot" that results from a plot of log k^sub f^ with [denaturant] is taken to indicate a change of transition state (and by implication the existence of an intermediate)
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.[FIGURE 11 OMITTED]DiscussionOvarian development for black rockfish in Oregon was similar to the developmental cycles reported for other rockfish species (Moser, 1967; Bowers, 1992; Nichol and Pikitch, 1994) with the exception of seasonal timing and stage duration
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. These rates are much faster than the overall rate constant for ribozyme docking observed in previous ensemble kinetic studies, which is on the order of 10^sup -2^ s^sup -1^ for a 2WJ ribozyme construct (N
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