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. Assuming an average lifetime of 3 ns for Trp in aqueous solution (Ferreira, 1989), the resulting value of K^sub SV^ for iodide quenching of Trp is 19
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. This choice is based on previous work (48)
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. Protein expression and purification The EI^sup sc^ clone comprises residues 1-576, with an extra methionine and a His^sub 6^-tag at the N-terminus (15)
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. The absence of a long native baseline at this and other pH in EI^sup sc^ precluded any reliable conclusion on the pH independence of this value of T^sub m^
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. ACKNOWLEDGMENTSSupport for this project from the Health Transitions Fund, Health Canada; the Canadian Health Services Research Foundation; Brogan Inc
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