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. The joint position of the changes in the two adjacent lines was around 68°C, suggesting the appearance of an intermediate at ~68°C
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. MATERIALS AND METHODS Materials Tris (hydroxymethyl) aminomethane buffer, [N-[2-hydroxyethyl]piperazine-N'-[2-ethanesulphonic acid]] (HEPES), and guanidinium hydrochloride were from Sigma Chemical (St
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. The peptide unit is always trails with ω = 179
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. The whole emission spectrum with a wavelength increment of I nm was acquired approximately within 30 s
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. Since small molecules cross the cell membrane by passive diffusion at a rate mainly determined by the molecule's hydrophobicity, which is commonly expressed in terms of the partition coefficient P between n-octanol and water (37,38), the permeability of the molecules can be estimated from calculations of the partition coefficients of the drug's components
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. Octanol-Water Partition Coefficients: Fundamentals and Physical Chemistry
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. RT^sup n^^sub 1^ only decreases below 1 when the internal motion is slower than 200 ps or when an additional internal motion with a timescale slower than 200 ps is present
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