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Accurate models for denatured states also impact a range of areas, including quantitative studies of in vitro folding pathways (10,19-24), protein design (25,26), studies of protein aggregation (27-29), and understanding preferential interactions in cosolute mixtures (1,3,30-36) bad collateral credit loan no .
Thus, weakening the azurin fold by the mutations leads to the creation of additional binding sites characterized by a broad distribution of binding affinities, a large fraction of which are of stronger affinity than in the WT protein bad collateral credit loan no .
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Single-pair FRET (spFRET) measurements can provide new insights into the details of RNA folding processes, by virtue of their enhanced abilities to report distributions and dynamics of molecular conformations (Ha et al, 1999; Zhuang et al bad collateral credit loan no .
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Received 13 January 2003; Accepted after revision 3 February 2004 bad collateral credit loan no .
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