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. Runs were carried out at 23°C on a Shimadzu (Kyoto, Japan) LC-10AS HPLC at a flow rate of 0
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. Cooperativity induced by a single mutation at the subunit interface of a dimeric enzyme: glutathione reductase
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. The obtained peak maxima around 336-338 nm indicate rather small red shifts (~2-3 nm) with respect to the native state (see also Fitter and Haber-Pohlmeier (14))
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. The final water content was measured by weighing shortly before closing the sample holders and was 0
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. 2 and then adding the probe at a 2:1 molar ratio
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. , 1999) to CPM-PLCβ gave a volume change similar to Gβγ-subunits (Table 1), which suggests that the contacts made by this peptide reflect the same ones made by the whole Gβγ-subunit (see Discussion)
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. Because this peak does not provide relevant FRET (distance) information, only the region above E = 0
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. The cooperativity coefficients (n) are all close to unity: 1
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- " All too often over the years--even decades--some councils have overridden the scientific conclusions and set catch limits that were too high
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. Since gSBA and rSBA both exist as tetramers with identical ligand binding specificities and macromolecular properties, the change of the mode of unfolding from a two-state process to a three-state process and the decrease of T^sub m^ by 26°C for the thermal unfolding of the protein of the kilter implicate the covalenlly linked glycans for the stability of gSBA
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. CALCULATION OF SCATTERING PROFILES In Eq
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.The maximum wetting forces for Sn-Ag-Cu-Bi solder in comparison with Sn-Pb solder are shown in Fig
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. Although the misfolding and aggregation reaction is very complex and of multiple order, all kinetic traces could be fitted to a double exponential function which enabled comparison of kinetic parameters
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. An advantage of this PLCβ construct is that it can be bacterially expressed (see Wang et al
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. The only other sympatric species of Pinguipedidae in the Argentine shelf is the Brazilian sandperch (Pinguipes brasilianus), which shares several similarities in meristic counts with P
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