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Spectral changes were found to be reversible upon removal of the denaturant by dialysis (data not shown)
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Five-hundred milligrams of holomyoglobin (purchased from Sigma, St
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Structural characterization of unfolded states of apomyoglobin using residual dipolar couplings
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The instrument was periodically calibrated with (+) 10-camphorsulphonic acid
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This shape did not change as the pH was modified, except in the region between 3
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Partition coefficient calculation The partition coefficients were calculated with the ClogP program (available at http://www
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The role of dynamics in enzyme activity
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9 (the root mean square (rms) error in ΔH^sup #^ is ~4 kcal/mol, based on the rms error in f of ~0
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We are grateful to Per Persson for initial assistance with electron microscopy work and to Jeffery Kelly and David Wemmer for providing the pmmHα plasmid
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Because our sample included all mature females that we encountered, we used these data to estimate the age distribution of mature females in each time period, and the age distribution of parturition during each time interval
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t] x t [[beta]
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