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. the intensity of the fluorescence spectra decreased (Fig
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. A similar difference in emission peak maxima between thermal and GndHCl-induced unfolding was also observed for other proteins (see for example (10,21))
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. In this context we wish to stress that, since IENS measures global mean atomic fluctuations (i
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. Notwithstanding these experimental results, we were not able to detect any differences in global MSDs between DmAChE and HuBChE, despite the former having a much lower degree of glycosylation than the latter
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. Results of osmotic squeezing at pH 3
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. The specific properties of Li+ ion have been discussed previously (Wolf et al
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. Sequence-specific binding of counterions to B-DNA
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. 5 mg/ml protein concentration was stored in the dark at 4°C and was used in four weeks, during which no protein degradation was detected
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. Therefore, throughout the following overnight dry incubation period no further structural transitions are likely to occur in the amyloid samples
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. To evaluate the effect of the protein concentration on the fluorescence spectra, a diluted sample solution with a final concentration of 0
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. ACYI contains eight Trp residues in each of its two subunits (47,48), and the eight Trp residues are distributed in both the peptidase and dimerization domain (13,17)
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. Sharmistha Sinha* and Avadhesha Surolia*[dagger] * Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India; and [dagger] National Institute of Immunology, New Delhi, India Submitted July 3, 2006, and accepted for publication August 29, 2006
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. In the last decade, strong binding of ANS to molten globule states of proteins has been linked to the loss of tertiary structure (7), and since then the method has been applied to characterize transient states in protein denaturation (8-11)
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. The latter feature shows that weak binding sites, similar to those induced in azurin by the destabilizing salt KSCN (12), are common with proteins under these conditions
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.1 ppm, and the aceturate peaks (a, d, and e) at 1
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. The principal function of acetylcholinesterase (AChE, EC
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. Tholen, Soldering Surface Mount Technol
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. In oscillatory shear experiments, the storage modulus crossed the loss modulus at concentrations of 54% w/w, an indication of the approaching glass transition
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. 5, C and D) and shorter fluorescence lifetimes (data not shown) reveal Trp to ANS and Bis-ANS fluorescence resonance energy transfer both in the native and misfolded states, indicating that the Tips and the probes are in close proximity (Conrad and Brand, 1968)
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. DCVJ was found to be very sensitive to formation of early oligomers and showed the strongest apparent binding affinity of the probes in the study
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., 2001; De Beer et al
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