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. In other words, although sequence specificity is not apparent in the average denatured-state topology, it is apparent in the way the symmetry characteristic of the denatured state is broken
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. Ice-induced ANS binding to other proteins To examine whether the ice-induced perturbation observed for azurin is common to proteins in general, ANS binding experiments were extended to a set of seven other proteins including monomeric BSA, α-amylase and β-gal, dimeric LADH and tetrameric ALD, YADH, and LDH
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