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Buffer contributions were subtracted, and the resulting spectra were used for analysis
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With respect to tryptophan residues, five spectral classes of emission spectra have been identified which exhibit significantly different emission peak maxima
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For all heating rates the secondary structure elements (mainly α-helices) fully disappear and compared to GndHCl-induced unfolding (data not shown here) the CD signals are identical
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After eliminating the denaturing force the proteins are not able to fold back into their native (and functional) structure and the still unfolded proteins form (or just stay on with) aggregates
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1 is used) assuming the apparent specific volume of the protein phase to be 1
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The theoretical equation of state does not describe the experimental results as well as at pHs 8
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Competitive electrostatic binding of charged ligands to polyelectrolytes: planar and cylindrical geometries
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A dramatic increase in the fluorescence quantum yield and a concomitant blue shift of the ANS and Bis-ANS fluorescence is evident upon binding to native TTR (Fig
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Because this peak does not provide relevant FRET (distance) information, only the region above E = 0
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5 and 6, and several corresponding pairs of rate constants (data not shown), and find these to be poorer descriptions of the experimental data as well
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Although Errington (1930) quantified the representation of bird bones in pellets, his samples were too small to draw conclusions, except to say that neither small birds nor mice were always represented in pellets in equal numbers to those that were ingested
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Immunolocalisation showed extensive staining for type-II collagen in pellets cultured in DMEM/ITS+/TGF-[beta]1 (Fig
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