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. On the other hand, tryptophan emission is quite sensitive to the polarity of the surrounding environment, and fluorescence red-shifts are indicative of increased exposure of tryptophan residues to the aqueous medium (Lakowicz, 1983)
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. To avoid misleading results from the heavy overlapping of the original IR amide I' band, FSD data, which maintained the intensity of the original data, were used for the phase diagram analysis
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. Interpretations suggesting that highly denatured proteins might he embryos of their native-state counterparts must he treated with extreme caution because there is no sound theoretical basis for such proposals
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. O'Donnell, MS, MBA, is chief editor of Prepared Foods/ NutraSolutions magazine
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. Furthermore, the K^sub sv^ values were higher than those measured at neutral pH but smaller than those at acidic pH and in 7 M GdmHCl (Table 1 )
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. To contribute to the ongoing discussion and to investigate the relationship among molecular dynamics, activity, inhibitor-binding, and unfolding, we carried out incoherent elastic neutron scattering experiments on two members of the cholinesterase (ChE) family: Tetrameric human butyrylcholinesterase (HuBChE), both native and covalently inhibited by the organophosphate nerve agent, soman (18), and dimeric native Drosophila melanogaster acetylcholinesterase (DmAChE)
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. Most importantly, as for Eqs
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. They can either be in fast exchange or in slow exchange to a lowly populated bound CaM state
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. The unfolding rate increases at higher temperatures, indicating high enthalpic contribution ΔH* to this barrier
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. The rationale of this work was to inquire into the hierarchical assembly of the insulin amyloid and link the morphological transitions to conformational events observed by other biophysical and spcctroscopic methods
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. Appending the complex trajectory of gp120 to the free one gives rise to a small increase (ΔS^sup 2nd,f+c^^sub CD4,F43^ = 0
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