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. Coupling of protein surface hydrophobicity change to ATP hydrolysis by myosin motor domain
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. The results in Fig
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. From the changes in the fluorescence intensity, the apparent pK^sub a^ of the titration was 4
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. Calreticulin has also been identified as another eat-me signal on apoptotic cells
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. ΔR^sub ex^ calculated using Eq
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. 6 kDa and a protein specific volume of 0
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. 75 ml g^sup -1^ was then used to obtain the mass of water in the hydrodynamic domain of the protein, and from this the volume fraction of protein at known mass fractions was estimated
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. The purity of stock GR preparations was routinely verified by SDS-PAGE, followed by Coomassie-Blue staining
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. The dominant crosspeak in the asynchronous plot was a positive peak centered at (1629 cm^sup -1^, 1617 cm^sup -1^), whereas minor positive peaks could he found at (1648 cm^sup -1^, 1617 cm^sup -1^) and (1658 cm^sup -1^, 1617 cm^sup -1^)
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. The asynchronous plot was different from that in Fig
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. Scale invariance applies to a variety of chain properties that go beyond chain size (39)
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. For example, at 20 µm ANS where binding sites are fully saturated in solution and the intensity has reached its maximum value, the corresponding intensity in ice is merely 3%-5% of the value in the liquid water
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. 2 and in Supplementary Material (Table S1)
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.RESULTSThermal transitions in lipid-free and in DMPC-bound apoA-2Far-UV CD data of lipid-free apoA-2 were recorded from 5 to 20 µg/ml protein solution in 10 mM Na phosphate, pH 7
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