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While the entrepreneur may plan to use a certain number of items over a period of time, their need often varies
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. parent company of Red Lobster and Olive Garden and a major seafood importer, has refused to bow to Protect Seals' demands
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. Taken together, these results suggest that at least two intermediate states can be identified in the equilibrium unfolding of GR, namely a partially unfolded predissociated state and a molten globule monomer
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. The dialyzed solution was centrifuged at 8000 rpm for 15 min and the clear supernatant was loaded on a lactosylamine Biogel P-150 column preequilibrated wilh buffer (21)
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. Lectin purification on affinity columns containing reductively aminated disaccharides
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. The development of an accurate EV limit description for denatured proteins mirrors the use of the hard-sphere fluid as a reference state for van der Waals liquids (51,52)
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. Interdomain interaction and substrate coupling effects of dimerizalion and conformational stability of Enzyme I of the Esclierichia coli phosphoenolpyruvate:sugar phosphotransferase system
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. averaged over three scans, and finally corrected for the buffer signal
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. MR images were acquired using a spin-echo sequence with simultaneous suppression of the lipid peaks between 0 and 3 ppm using three successive sinc3 pulses of 3 ms length and 2070 Hz bandwidth centered at 1
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. The aggregation process produced fairly monodisperse oligomers (300-500 kD) within 2 h that matured after 20 h into larger spherical clusters (30-50 nm in diameter) and protofibrils as shown by transmission electron microscopy
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. The observed tendency of untwisted lateral agglomerates (Fig
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. Calculation of the absolute molecular entropy would require a complete translational and rotational sampling
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. We identified feathers by direct comparison with museum specimens and by use of microscopic characteristics in the plumulaceous (downy) barbs (Sabo and Laybourne 1994)
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- She pockets a total $300 to $500 per month
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.Stone, who faithfully contributes 14% of her salary to her 401(k) plan and has $80,000 collectively saved toward retirement, is determined to make real estate investing an integral part of her investment strategy
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. The Mn^sup 2+^ and Ca^sup 2+^ concentrations used were 5 mM in all the experiments unless specified otherwise
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. For sequence separations that go beyond this local length scale, chain properties such as R^sub g^
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. Some efferocytosis receptors appear to perform primarily a tethering function by holding the apoptotic cell and phagocyte close together (eg, CD14 or CD31), (19,21,22) while other receptors are primarily involved with transducing the engulfment signal (eg, LRP and PS receptors [PSRs]), (11,21,23,24) hence the so-called tether-and-tickle hypothesis (Fig 1, top, A)
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. In contrast, RhoA induces the formation of stress fibers, focal adhesions, and cell spreading
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.Adaptive ImmunityThe strongest evidence linking efferocytosis with the regulation of adaptive immunity is the association of human and murine autoimmune diseases with the impaired removal of apoptotic cells
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. The two fibers marked with the green and blue arrows are of the comparable height of 6
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.30 BL; mean HL/BL=0
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. Assessment of the complete system was not yet feasible due to insufficient sampling, especially for gp120
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. In order to suppress non-specific binding of IgG, the sections were blocked with normal rabbit serum (Dako AJS, Denmark), diluted 1:5 in TBS for 20 minutes
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