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Thermodynamic puzzle of apomyoglobin unfolding
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Far-ultraviolet (UV) circular dichroism (CD) measurements were made in a 0
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In Flory's approach, a range of chain properties including R^sub g^, the average end-to-end distance (R^sub e^), the hydrodynamic radius (R^sub h^), the second virial coefficient (B^sub 2^), and the osmotic pressure (II) are calculated as series expansions in terms of the parameter z = N (T - Θ/T) (39,40,45)
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FTIR The protein was speed-vacuum dried (Speed-vac rotatory evaporator, Savant Instruments, Farmingdale, NY) and dissolved in the deuterated buffer containing 10OmM NaCI
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The helix-loop-helix motif formed by the α-helix 9-α-helix 10 shows intermonomer salt bridges: Asp^sup 540^ in the motif with Arg^sup 422^' in α-helix 5 from the other monomer (the prime is used to designate residues in the other monomer)
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All α-amylase structures bear multiple tryptophan residues more or less equally distributed over the whole structure (see Table 1 and Fig
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After 2 h, only very small aggregates (400 h), the protofibrils have increased in length to 100-200 nm, and the spherical clusters have decreased in size to 20-30 nm in diameter (Fig
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This probe becomes fluorescent when it reacts with cysteine side chains (see Molecular Probes Handbook, Eugene, OR)
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Although different distribution profiles of fibrillar features may still be explained in terms of kinetic effects (temperature may differently affect the kinetics at various stages of the assembly, effectively marginalizing certain sequential processes), the higher temperature may also have a more direct effect on amyloidogenesis, for instance by increasing the thermal energy of the interacting molecules and hence causing the alignment of protofilaments to become less accurate
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Nor does it anticipate specifically evolved favorable departures from the random imbalances of entropy and energy assumed here, which certainly arise and roughen the landscape, nor does it account for specific behavior from hydration shells
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MOLSCRIPT-a program to produce both detailed and schematic plots of protein structures
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The four IPP polymers differed in molecular weight, and the four EVA polymers had different vinyl acetate contents (EVA XX% designates XX weight% of vinyl acetate units, with the balance being ethylene units)
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Seasonal cycles of reserves in relation tc) reproduc- tion in Sebastes
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