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Nevertheless, the 2D IR analysis also suggested that the pretransitional conformational changes involved the unfolding of the solvent-exposed β-sheet structures and aggregates appearing at a temperature at which the protein had not fully unfolded
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All the experiments were done in a Jobin Yvon Horiba fluorometer (Jobin Yvon Spex, Cedex, France) in a 1-cm water- jacketed cell using a protein concentration of 1 µM and ANS concentration of 50 µM
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Information about the IMGC is available online at www
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Depending on the kind of denaturing conditions (high salt concentrations, extreme pH, elevated temperatures, pressure, etc
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GndHCl-induced unfolded states show for all α-amylases emission spectra with λ^sub max^ of ~348-349 nm which is nearly the value one would expect for fully accessible tryptophan, namely 350 nm
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5 ms for (200 µm)^sup 3^ voxel spectra
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Fournier,[double dagger] L
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The remaining residues (see Table 1) show a weak decrease in exchange rate upon change in temperature (f [asymptotically =] 0
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Internal condensation of a single DNA molecule
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This agrees with the DSC data from an identical sample indicating that disk reconstitution after heat denaturation takes more than 48 h to complete at 24°C (Fig
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Although the pigment appears to be well dispersed in solution, it may never have completely dispersed prior to the TIPS process
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Additionally, only this ribozyme is almost completely docked at physiological concentrations of Mg^sup 2+^ (~2 mM)
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