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5 and 3 M, and that a predissociated state with an expanded hydrodynamic radius compared to the native dimer is stabilized at 2
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Pézolet, and M
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The histogram of interresidue contacts can be plotted as a contact density map and the results are shown in the top row of Fig
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the development of a robust understanding of preferential interactions in cosolute mixtures (31-36)
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002 µg/µl for trypsin, that is, the final concentration rate between both proteins was 500:1) in a total volume of 17 µl in 100 mM of Tris buffer at pH 7 (27)
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Address reprint requests to Martin Weik, Institut de Biologie Structurale CEA-CNRS, 41 rue Jules Horowitz, F-38027 Grenoble Cedex 1, France
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For example, it has recently been shown that the simulated packing of ellipsoidal particles showed a dependence on aspect ratio and can approach a packing, φ^sub max^, of ~0
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Surface rheological properties of native and S-ovalbumin are correlated with the development of an intermolecular beta-sheet network at the air-water interface
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Although the dications studied showed no difference in their affinity for coil DNA, their compaction activities differed by 100-fold
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This indicates that one reason for the very strong quantum yield of ThT bound to amyloid fibrils is due to rotational relaxation
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Dipyrenylphosphatidylcholines as membrane fluidity probes
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The kinetic CD data were also recorded in denaturant-jump experiments with the disks
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In summary, both the kinetic (Fig
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, 2002); however, correlation of the CD and 90° light-scattering data in Fig
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, 2003), which are likely to consist of tubularly-stacked infinite β-helices (Jimenez et al
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Truncated variants of gp120 bind CD4 with high-affinity and suggest a minimum CD4 binding region
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Of the 30 cases of reactive mesothelial proliferation, 16 were biopsies from pleuropulmonary specimens (6 lungs with overlying pleura and 10 pleural specimens) and 14 were cell blocks from pleural effusions
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Both positive and negative controls were included during each test
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21 The supernatant was removed and the resultant pellet cultures were treated for 48 hours at 37°C/5%CO^sub 2^ in a defined medium comprising of Dulbecco's Modified Eagles Medium, ITS+ Premix (Collaborative Biomedical Products: insulin (6
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. We hypothesize that this compensatory effect could be due to a change in the coordination sphere of the Mg^sup 2+^ by directly involving the phosphoryl group of the PEP moiety, as it occurs in a member of the pyruvate dikinase family (57)
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. In soman-aged HuBChE, the organophosphoryl moiety remains covalently attached to the unfolded protein, and the salt-bridge between the protonated imidazole ring of His-438 and the negatively charged phosphoryl oxygens may contribute to the observed shift in the onset of unfolding to a higher temperature compared to the native enzyme
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. The S^sup 2^^sub s^ was either kept at a uniform constant value (M2I) or optimized together with Ï„^sub if^ and S^sup 2^^sub f^ (M2II)
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.5 × 10^sup -11^ m^sup 2^s^sup -1^, in agreement with previous measurements carried out under similar conditions and concentration (33)
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