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Interestingly, the results from fluorescence and infrared (IR) spectroscopy clearly indicated that pretransitional conformational changes occurred at temperatures from 50°C to 66°C during ACYI thermal denaturation
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As shown in Fig
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Importance of the carboxyl-lemiinal domain of enzyme I of the Eschericliia coli phosphocnolpyruvate:sugar phosphotransferase system for phosphoryl donor specificity
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However, more meaningful data might be obtained by employing encapsulation techniques, which in principle can ensure studies without interfering interactions of tryptophan residues with the encapsulating material
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020 [Angstrom], and the ^sup 15^N CSA value to -170 ppm
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4 ns, the angles Φ that the helices in CaM make with the main axis of the diffusion tensor were derived using Eq
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Consequently, they have a lower activation enthalpy of ~3
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When these previous reports (Table 1) are compared with our results on the DNA compaction potentials of monocations, dramatic differences are noted between the DNA binding and DNA compaction potentials of monovalent ions
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, upon various therapeutic treatments
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Additionally, little change in membrane polarity and hydration of POPC/PI(4,5)P^sub 2^ (67:33) membranes are detected in this same pressure range (Rebecchi et al
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After the buffer baseline subtraction, the data were normalized to protein concentration and expressed as molar residue duplicity, [Θ], No changes in [Θ] were detected with changes in the protein concentration in the range explored (5-600 µg/ml)
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Deep-hole drilling is a different operation requiring a different level of lubricity
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t] = 1] - E[[CostDayNSD
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ResultsOf the 152 participants surveyed, 80
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"I don't disagree [about] the convulsive effects that a strike would have
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ChauAspects of risk-taking behavior may be more or less amenable to formalized analysis (Trimpop, 1994; Yates & Stone, 1992)
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Split 4s only against a 5 or 6
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. Using these procedures, the energetics and pathways of folding, as well as the existence of transient intermediate states between unfolded and fully folded polypeptides, have been characterized for a number of proteins (for a collection of reviews, see Creighton, 1992; Pain, 1994)
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