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Besides, complex formation between GR and NADPH was only detected in the dimeric form of the enzyme, which would argue that NADPH does not participate directly in the process of GR folding/assembly
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The final enzyme concentration for the activity assay was 2 µM (~0
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It should be noted, however, that B-factors from x-ray crystallography, in general, include contributions from static disorder, and care must be taken in comparing them to thermal atomic fluctuations (14)
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In addition, there may be other factors such as specific planar excitation of ThT in a symmetric binding cavity that is required for the amyloid signal
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Transthyretin aggregation under partially denaturing conditions is a downhill polymerization
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, light scattering) or selectively detecting the amyloid fibrils (fluorescence of amyloids labeled with thioflavin T; e
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The energies were calculated every 5 ps as reported earlier by Hsu and Bonvin, 2004, and therefore N is 16,001 when analyzing the 2-10 ns simulation segments
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4), the entropy of most of the structural elements involved in the gp120/CD4 interface starts to level off after 4 ns as a result of their smaller number of atoms
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" Journal of Clinical Epidemiology 45: 187-95
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