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The fact that the CD thermal transitions of EI^sup sc^ became broad as the concentration was increased suggests that the thermal transition of the EIC^sup sc^ is also being observed, as happens in EIec (52)
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In both cases, the rate of increase of the MSDs decreases drastically at the highest measured temperatures (T > 270 K), as observed previously (44)
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We conclude that stabilization, and corresponding effects on the MSDs of HuBChE, by solvent/buffer and by the covalently bound inhibitor involve two different mechanisms
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