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Compared to native GR (circles), addition of 3 M GdnHCl (triangles) led to a significant increase in quenching efficiency, as indicated by an increase in K^sub SV^ from 12
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Thermal denaturation measurements Experiments were performed with a response time of 8 s and at constant heating rates of 15, 30, and 60 K/h to test for microreversibility (6), in the presence of 1 mM β-mercaptoethanol
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Blank corrections were made in all spectra
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whether the T^sub m^S are the same as at pH 7
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To aid comparing the ANS binding propensity among the various proteins Table 2 lists the minimum number of binding sites (n) as well as the ratio n/Mw, normalization by the molecular weight taking into account that larger macromolecules, such as oligomers, have a greater probability to bind ANS simply for their larger mass
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The lower value for the Bis-ANS case is (at least) partially due to the shorter lifetime that will truncate the measurement since the rotational correlation time is considerably longer than the decay lifetime
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The interaction was not significant, F (1,140) =
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