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Although we observe a qualitative proportionality between smaller red shifts and a significant aggregation of thermal unfolded states (at least for BAA, BLA, and BSUA), we can not exclude other and maybe additional mechanisms responsible for smaller red shifts during thermal unfolding as compared to those obtained with GndHCl-induced unfolding
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Molecular dynamics of acetylcholinesterase dimer complexed with tacrine
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Given an anisotropy of 2
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Furthermore, the nanosecond-timescale domain motion, present in free CaM, seems to be frozen out, and as far as we know, microsecond-timescale motions are not present in the wraparound binding (10)
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The fluorescence intensity of the different probes in the presence of the unfolded monomer state is shown in Fig
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Summary of the use of ANS, Bis-ANS, DCVJ, and ThT as fluorescence conformational probesThe results of the analysis of the fluorescence response in binding to unfolded monomers, native TTR, and various stages of the aggregation reaction is summarized in Table 2
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