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at both excitation wavelengths, showed a bell-shaped profile, with a maximum at neutral pH (Fig
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Furthermore, the K^sub sv^ values were higher than those measured at neutral pH but smaller than those at acidic pH and in 7 M GdmHCl (Table 1 )
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Similarly, collectin family members, including mannose-binding lectin, surfactant protein (SP)-A, SP-D, and the collectin-like Clq, opsonize apoptotic cells with their globular heads, and then form a complex through their collagenous tails with phagocyte calreticulin and LRP
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1 M potassium cyanide and 1 mM EDTA in 0
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The data points scatter around the line with k^sup -1^^sub ex^ [asymptotically =] 50 µs
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Further maturation of the aggregates showed shrinkage of the spheres as the fibrils grew in length, suggesting a conformational change of the spheres into more rigid structures
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Unlike PH-PLCδ^sub 1^, the membrane binding face and orientation of PH-PLCβ^sub 2^ are unknown
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The +4°C shift, the reduced height, and the increased width (decreased cooperativity) of this peak as compared to the sharp transition in DMPC vesicles at 24°C (Chapman, 1975) are characteristic of lipoprotein disks that have smaller co-operative lipid domains than vesicles
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This, as well as visualization of early unstructured aggregates, are the particularly advantageous features of AFM in studies on amyloidogenesis
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The model provides an alternative interpretation of the results of protein engineering analysis, and implies that not all contributions to measured Φ-values at the transition state may arise from native-like interactions
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5 and 6, and several corresponding pairs of rate constants (data not shown), and find these to be poorer descriptions of the experimental data as well
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((Comments on this story may be sent to info@m2
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