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We then "roll an N-sided die" to choose a residue whose torsion angles are to be altered
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CD measurements in the far-ultraviolet (UV) region Spectra were collected on a Jasco J810 (Tokyo, Japan) spectropolarimeter fitted with a thermostated cell holder and interfaced with a Peltier cell, working at 298 K
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In particular the wavelength of the tryptophan fluorescence emission peak (λ^sub max^) is a valuable parameter to characterize properties of the unfolded state
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The interfacial layer can form both at an air/water (54-57) and an immiscible liquid/ water interface (58,59)
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Dried lipids were suspended in 150mM NaCl, 20mM Hepes (pH 7
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, ≤1 kbar), we find that the volume change of PLCβ-Gβγ association is reduced
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Disk formation was confirmed by negative staining electron microscopy (EM) and non-denaturing gel electrophoresis which showed no free protein
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01 kJ K^sup -1^ mol^sup -1^ (solid line in Fig
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After the assay, positive reactions were observed in six wells
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The finding of a gender difference in the percentage of probable pathological gamblers is consistent with our previous results, but the failure to find a gender difference on the item of ever having gamble in a casino suggests that underage females are just as likely to enter a casino and gamble as under age males
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. This apparent K^sub d^, which reflects protein-protein associations on a confined membrane surface, is predicted to be ~2 orders of magnitude weaker when the proteins are free to associate in solution (see Runnels and Scarlata, 1999)
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