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. Too often, the language in this form has been preprogrammed into a document preparation system
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. Of the numerous roles credited to glycosylation the major ones are to facilitate proper folding, prevent protein aggregation, protect against proteolytic attack, and act as surface cell receptors and targeting signals (4,5)
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. rSBA showed no change in either the fluorescence emission profile or the secondary CD profile
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. All properties characterized from simulated ensembles match predictions from rigorous field theories
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. Validity of the "two-state" hypothesis for conformational transitions for proteins
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. Building better foods and supplements: consumer concerns about weight and interest in addressing specific health issues through diet bode well for research-supported milk proteinsJoseph A
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. 1 M^sup -1^) or both effectors (1
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. The catalytic turnover number of ChEs is among the highest known, approaching the diffusion limit (25)
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. According to the EDX analysis, neither Pb, Ag, nor Bi was detected along the solder/Cu or solder/Ni interface
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. 42 s^sup -1^ is obtained, which is very close to the average experimental R^sub 1^
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. On the other hand, globular proteins are generally nonspherical and there remain uncertainties in the prediction of interparticle forces
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. 1 in 10 mM acetate buffer and in this buffer containing 100 mM and 1
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. The concentration of Na ion fluctuates during the cell cycle, with peaks in the mitosis and synthesis phases, whereas the concentration of K+ does not change (Warley et al
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. Collapse of single DNA molecule in poly(ethylene glycol) solutions
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. 5 A, and see below)
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. The Θ^sub 222^(t) data show slow unfolding on a timescale of 10^sup 2^-10^sup 3^ s (as compared to 10^sup -8^-10^sup -10^ s for the helix-to-coil transition in solution; Yang and Gruebele, 2003), implicating high free-energy barrier ΔG*
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. , 2000; Rye and Barter, 2004; Jayaraman et al
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. The model also shows that high-dimensional structures provide conceptual relations between different models of protein folding
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. This is, however, rather prohibitive, considering the system size in this particular case
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. Excitation was achieved by focusing the 514-nm line of a 543-AP-A01 tunable argon-ion laser (Melles Griot, Carlsbad, CA) inside the sample solution, 10 µm from the glass coverslip surface, using a Plan Apo 100 ×, 1
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. In the final structure, the interface domain is hidden in a hydrophobic, packed core surrounded by the other domains, as demonstrated by crystallographic studies of human GR (Schulz et al
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. It has been proposed that the catalytic domain contains a β-sheet sandwiched between α-helices and a second β-sheet located on the surface, whereas the dimerization domain consists of a β-sheet flanked on one side by two α-helices (17)
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. The latter feature shows that weak binding sites, similar to those induced in azurin by the destabilizing salt KSCN (12), are common with proteins under these conditions
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. Dynamics of ANS binding to tuna apomyoglobin measured with fluorescence correlation spectroscopy
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. Briefly, we find: i), the observed 2
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. Although the blank fluorescence for DCVJ is low, DCVJ in the presence of unfolded TTR shows even lower fluorescence than the blank, which could indicate a small quenching effect through binding
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. Being aware that the rotational correlation time of the aggregates were larger than the fluorescence lifetime of bound ANS, we generated a distribution of aggregate sizes using a variety of TTR concentrations from 2 to 8 µM (monomer concentration) that were incubated for 20 h (Fig
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. were recorded at 222 nm during sample heating and cooling from 5-98°C at various scan rates from 1
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., the thickness of the DMPC bilayer) is 5
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