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. These folding intermediates exhibited flexible tertiary structures and nativelike secondary structures
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. A significant intensity decrease was observed for most IR bands from native secondary structures, accompanied with the exposure of most Trp residues to water and the formation of aggregates during the I [arrow right] A transition
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. The HuBChE samples were assayed before and after neutron scattering experiments by the Ellman procedure (36), with butyrylthiocholine iodide (1 mM) as the substrate
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. Financial support from the Deutsche Forschungsgemeinschaft and the Fonds der Chemischen Industrie and the COST D30 action "High Pressure Tuning of Biochemical Processes: Protein Dynamics and Aggregation" is gratefully acknowledged
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. After removing the PBS, 200 [micro]L of the hybridoma culture supernatant solution was added to the wells and incubated at room temperature for 30 minutes
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. As the temperature was increased, the intensity of the hands from the native secondary structures decreased, and two new bands (1618 and 1682 cm^sup -1^) appeared at temperatures above 70°C
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. Formally, folding can be viewed as a symmetry-breaking operation wherein the dilatation symmetry characteristic of the denatured-state (EV limit) ensemble is broken by breaking or disrupting the requisite number of nonnative local contacts
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. Similarly to that observed for EIC^sup ec^, the electrostatic, as well as the van der Waals interactions, were decreased in EIC^sup sc^, losing several salt bridges and hydrogen bonds when compared with the structure of EIC^sup tt^
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. As a representative for the first class, BLA shows a λ^sub max^-value for the native state of 333 nm, which increases up to 339 nm upon thermal unfolding
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. Before initiation of the aggregation reaction, TTR is in the form of unfolded monomers
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