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Toward a taxonomy of the denatured state: small angle scattering studies of unfolded proteins
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And finally, thermal denaturalions were carried out to test for changes in protein stability upon binding to the effectors
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However, at the relatively high CaM concentration (1 mM) used in the NMR experiments, compared to the nanomolar dissociation constant of the complex, all CaM is in the bound state
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Compaction curves for T4 DNA compaction in PEG 3000 solution induced by LiCl, NaCl, KCl, RbCl, and CsCl are shown on Fig
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For example, a sample that was used to record curve 1 in Fig
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, 2004), has been drawing attention to amyloidogenic pathways and structures of non-native protein assemblies
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In more biochemical language these structures might be exemplified by cooperative secondary structure formation alongside native or near-native distant contacts in α-helix bundle proteins (Myers and Oas, 2001), or simultaneous folding and anion binding (Henkels et al
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7 mm, were used to describe larval development
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5), 100 mM NaCl (referred to as annealing buffer) at a concentration of 1 µM donor strand, 5 µM acceptor strand, and 10 µM of additional strands (if applicable) and were annealed by heating to 70°C for 2 min and cooling to ambient temperature
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Blackjack glossary* Bankroll--The stake (available money) a player plans to bet with
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