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. This analysis showed a single protein band in the GR preparations used, indicating that the protein was essentially pure
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. Compared to native GR (circles), addition of 3 M GdnHCl (triangles) led to a significant increase in quenching efficiency, as indicated by an increase in K^sub SV^ from 12
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. Since the MG conformation is more flexible than a fully folded polypeptide, this could lead to better packing at the dimer interface, leading to more stable dimers
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. (3,7-9)We propose that the increased presence of apoptotic cells in patients with many of these chronic inflammatory lung diseases is largely due to impaired cell removal mechanisms
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. The binding of soman to HuBChE via a single covalent bond (plus a salt bridge between a negatively charged oxygen on the phosphoryl moiety, and the protonated imidazole ring of His-438) may explain why it does not noticeably affect global HuBChE molecular dynamics
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. In total, 116 residues were analyzed using the PINATA method (22), which is particularly suited for proteins that exhibit extensive nanosecond-timescale internal motions
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. Ovary connective tissue was retained in the coarse sieve, and freed eggs were collected in the fine-mesh sieve
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. This, as well as visualization of early unstructured aggregates, are the particularly advantageous features of AFM in studies on amyloidogenesis
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. Several general predictions result: 1), intermediate subspaces of configurations will always be visited; 2), specific but nonnative interactions may be important in stabilizing these low-dimensional diffusive searches on the folding pathway, as well as native interactions; 3), sequential barriers will commonly be found, even in "two-state" proteins; 4), very early times will show characteristic departures from single-exponential kinetics; and 5), contributions of nonnative interactions to Φ-values and "Chevron plots" are calculable, and may be significant
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