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For Trp residues in proteins, it is not uncommon to find average lifetimes that are significantly longer than for free Trp
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Parameter A, which reflects the spectral shape of the intrinsic Trp fluorescence (31), was obtained by dividing the fluorescence intensity at 320 nm by the intensity at 365 nm
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7 kcal mol^sup -1^ in terms of free energy of unfolding (27)
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Interestingly, the scaling exponent y [asymptotically =] 0
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CONCLUSIONSA series of incoherent elastic neutron scattering experiments were carried out on native and soman-inhibited human butyrylcholinesterase, as well as on Drosophila melanogaster acetylcholinesterase, to investigate the influence of irreversible chemical modifications (active-site phosphorylation) and structural differences (glycosylation, oligomeric state) on protein molecular dynamics
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The influence of small differences in primary and quaternary structure and in degree of glycosylationDifferences in primary and quaternary structure, degree of glycosylation, and oligomeric state, between DmAChE and HuBChE, although they affect various structural and functional parameters, are not reflected in changes in global dynamics on an Ångström-nanosecond scale in the temperature range investigated
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1 is used) assuming the apparent specific volume of the protein phase to be 1
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Heat-induced gelation of globular proteins
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The rationale of this work was to inquire into the hierarchical assembly of the insulin amyloid and link the morphological transitions to conformational events observed by other biophysical and spcctroscopic methods
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Interestingly, a closer scrutiny of the insulin aggregates obtained after 1-min incubation at 60°C indicates that clusters of swollen insulin particles tend to align within the immediate vicinity of the early fibers (Fig
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5-, 1-, and 2-ns block sizes) were used for fitting the standard errors to an exponential function using the xcrvfit software (http:// www
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