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. This process may be particularly important in the case of neutrophils, because of their large numbers during acute inflammation, their short lifespan, and their internal stores of proteases, inflammatory mediators, and oxidants
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. Thermal denaturation of Bungarus fasciatus acetylcholinestcrase: is aggregation a driving force in protein unfolding? Protein Sci
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. Water suppression was achieved by a weak presaturation pulse of 2
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. The static magnetic field dependence of chemical exchange linebroadening defines the NMR chemical shift time scale
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. Some extrinsic dyes that by molecular recognition bind to conformational substrates such as native, misfolded monomers and/or aggregates of TTR have been investigated in this work
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. It should however be noted that Bis-ANS still fluoresces very strongly in the presence of large aggregates (Figs
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. This clearly reduces the folding time enormously, signifying that only a tiny fraction of possible states is visited in the search (Dinner et al
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. Interestingly, for CD4-F43, the corresponding value is negative, -0
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. Folding of the four-way RNA junction of the hairpin ribozyme
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. It is known that apomyoglobin is stable in its native state at neutral pH and 30 deg C
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. In conclusion, our approach suggests that thermodynamical analysis might reveal subtle contormational changes in otherwise similar folds
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. Janssen; unpublished data; March 2006)
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. Alterations in ^sup 1^H NMR peak levels from intracellular lipids have been reported in various cell biological processes, such as cell activation, proliferation, and apoptosis (31)
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. The angle that a helix axis makes with the main axis of the diffusion tensor can therefore be determined with reasonable accuracy from (τ^sup o^^sub m^)^sub app^ averaged over a helix
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. We employed the DCVJ probe to investigate its potential use for recognition of misfolded aggregates formed from TTR
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. This is confirmed by EM, showing that similar thermal treatment leads to more extensive fusion of DMPC complexes with apoA-2 (Fig
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. The upper stripe developed from the tip of the snout and the lower one began below the eye, both extending to the anterior caudal peduncle
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. Temperature values in parentheses indicate thatonly surface temperature was registered
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.ResultsOf the total number of participants in the study, 76
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