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The influence of high hydrostatic pressure on structure, function and assembly of proteins and protein complexes
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Computational studies on the glycan-protein interactions at the noncanonical interface of the protein show that quite a number of hydrogen bond and hydrophobic interactions stabilize the glycoprotein tetramer
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Table I lists relevant information for the 23 protein sequences (68) used in this study
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EI proteins are composed of two domains (6,7): a proteolytically inert N-terminal (EIN), and a proteolytically unstable C-terminal domain (EIC)
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Biochem Biophys Res Commun 2004: 318: 32-37(52) Huynh ML, Malcolm KC, Kotaru C, et al
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Thus, we confined our study to a cell system with intracellular compartments larger than the smallest voxel dimension we could realistically obtain
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MSDs of native and soman-inhibited HuBChE were identical below the thermal denaturation temperature of the native enzyme, indicating a common mean free-energy surface
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Interfacial rheology of globular and flexible proteins at the hexadecane/water interface: comparison of shear and dilatation deformation
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, 2000, 2001; Kung and Reed, 1989) and in studies of the G-F transformation of actin (Sawada et al
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Very recently, the role of nonnative interactions in stabilizing an on-pathway intermediate, together with a diffusion-collision kinetic route, has been experimentally verified in the case of the immunity protein Im7 (Capaldi et al
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After the assay, positive reactions were observed in six wells
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Thus, it becomes possible to identify P
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These fundamental aspects of RNA folding are frequently obscured by the strong tendency of RNA to form alternate secondary or tertiary structures, which act as kinetic traps and retard the overall rate of folding
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A threshold of 40 counts (sum of signals from the two channels) was then used to separate fluorescence signals from background, and FRET efficiencies were calculated for each accepted event using the equation E = (1 + [gamma]I^sub d^/I^sub a^)^sup -1^
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