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. A further increase in quenching efficiency was observed at 7 M GdnHCl (squares, K^sub SV^ = 40 M^sup -1^), indicating that even at 3 M GdnHCl the tryptophan residues of GR were still significantly protected from iodide quenching relative to the fully unfolded protein
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. A similar principle underlies the design of energy landscape theories for folding kinetics that are based on Go models (105-107)
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. Moreover, the strategies used in this research might also facilitate the characterization of the potential pretransitional changes in the thermal transitions of other thermostable or thermophilic proteins
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. Although gSBA was seen to demonstrate very high conformational stability, rSBA exhibits comparatively much lower firmness to resist the structural perturbation induced by the denaturant guanidinium hydrochloride
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.INTRODUCTIONMany diseases are caused by protein misfolding
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. The physicochemical reason for ThT fluorescence in the presence of amyloid fibrils is not known (LeVine, 1993)
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