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The thermal unfolding of ACYI might be a three-state process involving an aggregation-prone intermediate appearing at ~68°C bank loan sba .
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For experiments involving PEP, control scans were acquired with PEP added to buffer versus buffer in the reference cell, and baselines were found to be the same as in buffer-buffer baselines, suggesting that under these conditions hydrolysis of PEP during heating was not significant bank loan sba .
This timescale corresponds well with the expected motions of the N- and C-terminal CaM domains bank loan sba .
The fluorescence intensity was registered by integration of the spectra to avoid adverse effects by spectral shifts bank loan sba .
Taken together, these data suggest that in solution PLCβ-Gβγ dimers further oligomerize to a higher order species such as tetramers bank loan sba .
33 K/min (80 K/h) yields similar CD melting curves (not shown), with the apparent T^sub m^ = 84°C identical to that of intact disks bank loan sba .
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Simple model of protein folding kinetics bank loan sba .
Egg and larval distributions of walleye pollock Theragra chalcogramma in the Shelikof Strait, Gulf of Alaska bank loan sba .
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