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If a fish living in a surface stream suffers a mutation harming its vision, it is more likely than its sighted fishmates to die of hunger or to be eaten by predators
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However, since folding requires the formation of spatial, long-range contacts, local nonnative contacts have to he broken
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How native-state topology affects the folding of dihydrofolate reductase and interleukin-1β
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The secondary structure of EI^sup sc^ upon binding was not largely affected (Table 4), but there were subtle changes in the stability of the protein (Table 3)
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However, in the CaM:SEF2-1mp complex, the two basic peptide arms are trapped in the hydrophobic interior of the CaM dimer
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Cytoplasmic concentrations relevant to macromolecular crowding are typically up to 40% v/v (72)
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Native tetrameric TTR can serve as a reference state to which some, but not all, fluorescence probes used in this study can bind
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. Since IENS averages 730 ps in our experiments over the total instrumental resolution time, it is possible that putative dynamical differences of opposite sign, on shorter timescales of ~10 ps, might simply have been cancelled out
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. An upper limit to the standard error at equilibrium can then be estimated by fitting the block average standard errors as a function of increasing trajectory block sizes to a single-exponential function
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