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the changes observed should reflect conlormational rearrangements occurring in this region
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Simulations of protein folding and unfolding
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Thermal behavior of human plasma high density lipoprotein
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The AFM-samples were dried gently with a nitrogen flow and left to dry overnight before the measurements, which helps improve the quality of the AFM data
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- "Mulford stands by his analysis
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. Trypsin digestion experiments EI^sup sc^ was mixed with trypsin (al linal concentrations of 1 µg/µl for El^sup sc^ and 0
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. To test whether there is a relationship between the extent of ANS binding induced by freezing and the instability of azurin to the freeze-thawing process, we have determined the degree of irreversible denaturation of WT and mutant species when subjected to repeated freeze-thawing cycles
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. Such a link between the instability to freeze-thawing and propensity to bind ANS in ice supports the notion that irreversible damage to freeze labile proteins, often resulting in enzyme inactivation and or protein aggregation, is likely to have originated from partial unfolding of the globular structure in ice
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. Strambini, unpublished data) provides additional evidence that the creation of these low affinity ANS binding sites is owed to perturbations of the protein fold associated with the frozen medium
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. This has been exploited in diffusion-weighted MRS to differentiate between intracellular and extracellular signal contributions of nonwater molecules (17,18)
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. In the vegetal cytoplasm, the aceturate peaks are still smaller than in the animal cytoplasm, concordant with the vegetal cytoplasm being even more stuffed with maternal macromolecules (31,39,40)
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. Secondly, the relaxation-compensated CPMG experiment (20) was employed at 500 and 600 MHz to measure ΔR^sub ex^
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. Counterion-induced condensation of deoxyribonucleic acid: a light-scattering study
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. ANS and DCVJ show significant fluorescence after l min
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. A total of 592 ichthyoplankton samples and 277 juvenile trawl samples were analyzed (Table 1)
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