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Identification of distinct length scales SAXS (62,63) and small-angle neutron scattering (64) measurements are useful for quantifying the average sizes, shapes, packing densities, and presence of distinct length scales in polymeric solutions
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The transitions measured by CD became broader as the concentration increased, and although a small increase was observed when the concentration was raised (3 µM yielded T^sub m^ = 328
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2 can at best provide a rough indication of the thermodynamic parameters governing ANS binding
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Structural dynamics of liganded myoglobin
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Modeling work was also carried out to compare with the experimental results
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Results in this study show that the use of ANS fluorescence intensity as a probe of molten globule formation can be complemented with fluorescence anisotropy decay to indicate the quaternary state of the molten-globule protein
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ORIGIN software was used for the data collection, analysis, and display
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This suggests that the protein refolding detected by CD between 32 and 24°C upon relatively rapid cooling (Fig
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