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According to this view, the tight and specific association of subunits in the native GR dimer may be facilitated by contacts between MG monomers
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There are two terms in the expression for the mean-field free energy, which is parameterized in terms of R^sub g^
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Details of these tests for convergence of the simulations and the sample size are presented in the Appendix
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The experimental results indicate that at physiological pH, the dimeric EI^sup SC^ had a well-folded structure; however, at low pH, EI^sup SC^ showed a partially unfolded state with the features of a molten globule, as suggested by fluorescence, far-ultraviolet circular dichroism, FTIR, and 8-anilino-1-naphthalene-sulfonic acid binding
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Among a large variety of different methods, such as absorption-, circular dichroism spectroscopy, calorimetry, NMR, and light scattering techniques, in particular fluorescence spectroscopy is a powerful technique to monitor unfolding transitions and to characterize properties of the unfolded state (see for example (3,4))
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5 ms and two 180° pulses of 0
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Glycoforms modify the dynamic stability and functional activity of an enzyme
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All results were tit to a binding isotherm after correcting for dilution and background scattering
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These may or may not be identified as intermediate states, depending on their occupancy lifetime
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. This indicated that most of the Trp residues in ACYI were buried in the interior (Class I) or exposed to bonded water (Class II), and no Trp residues were in a highly hydrophobic (Class A and S) or fully solvent-exposed (Class III) microenvironment
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. Here, T is the desired temperature and Θ is the theta temperature, where polymers behave like ideal chains, and N is the chain length
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. Thermal scans were followed by changes in the ellipticity at 222 nm from 298 to 353 K
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. The phosphotransferase system of Streptomyces coelicolor is biased for N-acetylglucosamine metabolism
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. The pronounced blue shift for native proteins in NDSB solutions is most probably related to the fact that NDSB interacts with the surface of the proteins which causes a more hydrophobic nonpolar environment of surface exposed tryptophan residues
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. Members of the ChE family differ from each other in turnover number
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. The binding of soman to HuBChE via a single covalent bond (plus a salt bridge between a negatively charged oxygen on the phosphoryl moiety, and the protonated imidazole ring of His-438) may explain why it does not noticeably affect global HuBChE molecular dynamics
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. Although the protein globule may carry a zero net charge, its surface charge distribution is heterogeneous and the net charge reflects the balance between positive and negative charges
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., 1974) as well as a function of water activity from 18
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. Furthermore, information on the effect on folding rate of replacing specific residues via mutation or "Φ-value" analysis (Fersht, 2000) needs to be taken together with correlations of contact order
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. The corresponding conformational entropy differences with their respective standard error estimates from the block averaging analysis are summarized in Table 2
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