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. The measured hydration number of U^sub A^ was close to that of the accessible water number (1340) of a protein molecule calculated for a fully extended structure, indicating that the structure of U^sub A^ is extended but somewhat more compact than that of a fully extended state
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. J Immunol 2000; 164:1663-1667(55) Karp CL, Flick LM, Park KW, et al
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. For example, problems arise from the intrinsic temperature dependence of the tryptophan fluorescence emission and from a pronounced protein concentration dependence, which leads to a significant scattering in the data
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. For the analysis of the relaxation data, the apparent overall tumbling time is an important parameter and we briefly describe the relevant equations
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. Although the differences in the χ^sup 2^ residuals between M2II models with different τ^sub is^ are not statistically significant, it is safe to conclude that there is a slow timescale motion present with a correlation time of roughly 2
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. The second virial coefficient is negative in this pH region, indicative of there being short-ranged attractive forces
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. 0, when the protein carries a net negative charge, can be described by hard sphere behavior those at pH 3
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. Apolipoprotein A-II inhibits high density lipoprotein remodeling and lipid-poor apolipoprotein A-I formation
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. Clearly this coarse-grained level of model cannot pick up specific and discontinuously large shifts of the transition state, which in small proteins will often dominate particular cases
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