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The changes of thermodynamic parameters (enthalpy change ((Delta)H), entropy change ((Delta)S) and heat capacity change ((delta)C^sub p^)) corresponding to the transitions from N to MG (molten globule state) or MG to U (unfolded state), were summarized in the previous report (Nishii et al
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Reversibility of the thermal transition was examined by reheating the samples after cooling from the first scan
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Further, the unfolding pathways in the two are widely different
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and thus, it was dominated by the emission of the sole tryptophan residue
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4 ms were applied in a point resolved spectroscopy sequence (PRESS) (26), along with spoiling gradients of 1 ms and 100 mT/m between pulses, which led to a minimum echo time of 6
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The availability of intracellular spectra opens a new window for the investigation of intracellular processes, since the spatial and temporal fingerprints of nonwater molecules, be they physiological cellular components or external substances, can directly be followed on a subcellular scale
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Estimation of water content and water mobility in the nucleus and cytoplasm of Xenopus laevis oocytes by NMR microscopy
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5) are identical for the folded native and soman-inhibited enzymes
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This makes a direct determination of the rotational correlation time impossible
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The molecular details of how PLCβ interacts with Gβγ is unknown
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7 B); T-jumps to other temperatures show a similar trend
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This information is needed to locate main reproductive and nursery grounds for the species
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Not only is its flexibility reduced, but its configuration is also altered
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These results are consistent with those obtained in a study conducted by Damush et al
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