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.Many species, of course, have lived in the total darkness of the underground for millennia, functioning perfectly well without vision
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.2 and 1 mg/ml were prepared and used for the Trp fluorescence analysis
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. To aid comparing the ANS binding propensity among the various proteins Table 2 lists the minimum number of binding sites (n) as well as the ratio n/Mw, normalization by the molecular weight taking into account that larger macromolecules, such as oligomers, have a greater probability to bind ANS simply for their larger mass
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.The plots can be interpreted in analogy to Guinier plots of polydisperse dilute solutions: In SAS experiments, linear regimes can be assigned to populations of distinct radii of gyration R^sub g^, provided Q*R^sub g^ Whereas at low temperatures, intensities decreased linearly over the whole Q-range measured for all samples, a more or less pronounced kink appeared in the Guinier plots above ~200 K (Fig
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. Hydration of pectic polysaccharides
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. One possibility was deactivated Gα-subunits that could displace Gβγ from PLCβ^sub 2^, but the addition of a large molar excess of Gα^sub i^(GDP)-subunits did not affect either the on- or off-rates
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. The "correctly folded" state of proteins: is it a metastable state? Angew
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. This will be the more likely as secondary structure forms, as we shall see by the example of a three-helix bundle below
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., 1995), and in October within San Matias Gulf (Gonzalez, 1998)
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