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. The fitting of the fluorescence spectra was performed using the discrete states model of Trp residues in proteins and was calculated by a program developed in-house (30) based on the SIMS algorithms of decomposition (32)
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. The Saccharomyces cerevisiae nucleoporin Nup2p is a natively unfolded protein
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. Quantitative measurement of protein stability from unfolding equilibria monitored with the fluorescence maximum wavelength
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. Doubly distilled milliQ water was used throughout
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. The sensitivity of the ANS binding method to the tertiary structure of proteins in ice should find practical utility in devising safer freeze-drying protocols of pharmaceutical proteins and testing the effectiveness of various stabilizing/ destabilizing agents employed in protein formulations
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. Source of variationdf Sum of squaresMean squareAbsolute fecundity (basedon prefertilization oocytes)Equality of slopes 2105,34752,674Error1604,564,32028,527Equality of elevation2168,54484,272Error1623,459,048 288,254Relative fecundity (basedon prefertilization oocytes)Equality of slopes 2 259
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. The resulting histogram can directly reveal conformational subpopulations that are averaged in conventional ensemble FRET measurements
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. Journal of Zoology 192:283-293
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.1°C/min reveals for all protein concentrations λ^sub max^-values around 345 nm for the unfolded state
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.[FIGURE 6, 7 & 8 OMITTED]Similar to the ANCOVA results for abso-lute fecundity, there were no differences inslopes or elevations for relative fecundity forprefertilization females for the years 1996-98 (Table 4)
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