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Phase diagram analysis of IR and fluorescence data The phase diagram analysis, which is a sensitive tool to detect folding intermediates, was carried out as described previously (35)
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The N [arrow right] I transition, which was referred to as the pretransitional change, ended at ~68°C and the T^sub 1/2^ occurred at ~60°C (see Table 1)
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Phophorylation destabilizes the amino-terminal domain of enzyme 1 of the Escherichia coli phosphoenolpyruvate:sugar phosphotransferase system
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Am J Respir Crit Care Med 2001; 163:737-744(4) Spalding KL, Bhardwaj RD, Buchholz BA, et al
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J Biol 2004; 3:15(32) Kinchen JM, Cabello J, Klingele D, et al
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); the Swedish Research Council for Engineering Sciences/SSF (T
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In a study that focused on the interaction between PLCβ^sub 2^- and Gβγ-subunits, we found, not surprisingly, that the measured steady-state dissociation constant for the two proteins was equal to the ratio of the on- and off-rates (Runnels and Scarlata, 1998)
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We note that we have found that the affinity between these proteins does not depend on the lipid phase (L
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[1] were (1) nucleation rates for LLPS and crystallization, (2) relative position of LLPS and crystallization temperature, (3) time provided by the thermal cycle for nucleation of phases during LLPS before nucleation of crystallization occurs, (4) relative positions of spinodal and binodal regions (nucleation rate of LLPS phases), and (5) growth of phases after nucleation (especially LLPS [1, 10, 15])
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* Point Count--The net value of the card count at the end of a hand
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