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. Glycosylation:heterogeneity and 3D structure of proteins
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. Folding mechanism of the CH2 antibody domain
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. For example, problems arise from the intrinsic temperature dependence of the tryptophan fluorescence emission and from a pronounced protein concentration dependence, which leads to a significant scattering in the data
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. This is particularly intriguing, since its three-dimensional structure reveals that the active site of AChE, which, like other serine hydrolases, features a Ser-His-Glu catalytic triad, is buried at the bottom of a deep and narrow gorge lined by aromatic residues (26)
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. Since γ^sub SL^ is solely a function of the solder-substrate interface metallurgy and is insensitive to the flux,14 the contact angle mainly dominated by the type of flux is pronounced
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. 3, we tested internal motion models with either one (M1; τ^sub 1^ and S^sup 2^ fitting parameters) or two contributions (M2; τ^sub if^, S^sup 2^^sub f^, τ^sub is^ and S^sup 2^^sub s^ fitting parameters) to the internal motion
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. ThT fluorescence at pH 1
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. Calorimetric denaturations were reversible in all cases
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. Control experiments were earned out at 7 M GdmHCl, pH 7, where the protein was fully unfolded
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. Opposing effects of phosphoenolpyrm ate and pyruvate with Mg^sup 2+^ on the conformational stability and dimerization of phosphotransferase enzyine I from Excherichia coli
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.4 ms was used for (180 µm)^sup 3^ voxel spectra, and TE = 6
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. Consequently, (τ^sup o^^sub m^)^sub app-corr^ may contain contributions from these slow internal motions and must be considered as an effective overall tumbling time
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., at different populations of free and bound CaM
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. In the case of the SL 4WJ, for the broad/asymmetric peaks at some of the high Mg^sup 2+^ concentrations, these fitted Epeak values are representative of the peak positions, but are higher than the average FRET efficiencies by ~0
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.1 is displayed in subsequent histograms
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