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Unfolding of GR by GdnHCl was further characterized by intrinsic fluorescence anisotropy measurements (Fig
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This solution was then dialyzed against 1
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10 for three proteins of different lengths
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The PTS system of Streptomyces coelicolor: identification and biochemical analysis of a histidine phosphocarrier protein HPr encoded by the gene ptsH
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The exchange broadening must then be due to conformational exchange between different conformations of bound CaM
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Information on the dynamics of protein hydration may be obtained from ^sup 17^O magnetic relaxation dispersion measurements, which indicate that water molecules at protein surfaces are highly mobile (66,67)
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Time-resolved fluorescence anisotropy decay of ANS was shown to be a useful tool for giving further structural and kinetic information of the oligomeric aggregates
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To enable characterization of different conformational states of TTR, we employed four different molecular probes: ANS, 4-4-bis-1-phenylamino-8-naphthalene sulfonate (Bis-ANS), 4-(dicyanovinyl)-julolidine (DCVJ), and thioflavin T (ThT) (Fig
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The compact mature fibrillar structures formed at the endpoint of the aggregation process may hence result from an effort to minimize the exposure of hydrophobic residues
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Interactions among α-synuclein, dopamine, and biomembranes: some clues for understanding neurodcgeneration in Parkinson's disease
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The percentages of positive stations were 5
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Free energy determination of polypeptide conformations generated by molecular dynamics
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5-ms time bins that we have used for the FRET histogram data
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